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Thermo Fisher
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Fisher Scientific
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Image Search Results
Journal: Journal of Virology
Article Title: Mechanical Barriers Restrict Invasion of Herpes Simplex Virus 1 into Human Oral Mucosa
doi: 10.1128/JVI.01295-17
Figure Lengend Snippet: Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by TrypLE Select treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Article Snippet: Cell suspensions prepared by
Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Staining, Standard Deviation